We have developed DNA/RNA synthesis equipment that is optimized for synthesis at the medium scale (10's of mgs to grams). Our synthesizer, the Q Master, features multiple monitoring modes, including colorimetric, gravimetric, and conductivity to ensure control and feedback of the synthesizer.
Using our unique trityl monitor control and feedback system, we can deliver varying volumes of DCA solution to accommodate the different deprotection times of bases as well as lengthening deprotection times with longer oligos. Trityl control and monitor minimizes exposure to acid by optimizing the amount of acid delivered at each base. By delivering only what is required we maintain tight controls of cost for this reagent. By optimizing acid delivery we achieve complete deprotection which results in optimized step wise coupling efficiencies and yields. With our ability to control acid delivery we do not compromise synthesis protocols.
Gravimetric control provides for positive verification of the delivery of reagents thereby ensuring built-in monitoring of critical reagents. This feature also controls costs which we can pass on the savings to you. In addition, it satisfies requirements for cGMP documentation.
Turbulent Flow: The Q Master uses turbulent flow for reagent delivery rather than laminar flow. Turbulent flow is preferable to laminar flow as it encourages mixing while laminar flow, which is used by HPLC pumps, is fine for HPLC but is not ideal for chemical synthesis which requires mixing. The Q Master uses a gear pump to achieve the turbulent flow affect.
Validated Software: The Q Master measures the delivery of critical reagents for a "closed loop" validation of operations, important for government documentation requirements.
The Q Master DNA / RNA
Medium Scale Synthesizer